Cloned (Comment) | Organism |
---|---|
genes bchX and bchY, recombinant expression of GST-tagged BchX and BchY in Escherichia coli | Rhodobacter capsulatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
8-vinyl chlorophyllide a | inhibitory effect of 8 V-Chlide on 3 V-BChlide production inhibitory effect of 8 V-Chlide on the C7=C8 reduction of COR | Rhodobacter capsulatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0014 | - |
8-vinyl chlorophyllide a | recombinant enzyme BchX/BchY, pH and temperature not specified in the publication | Rhodobacter capsulatus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Rhodobacter capsulatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-acetyl-3-devinylchlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + ATP + H2O + 2 H+ | Rhodobacter capsulatus | - |
bacteriochlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster + ADP + phosphate | - |
? | |
3-acetyl-3-devinylchlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + ATP + H2O + 2 H+ | Rhodobacter capsulatus NBRC 16581 | - |
bacteriochlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster + ADP + phosphate | - |
? | |
3-acetyl-3-devinylchlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + ATP + H2O + 2 H+ | Rhodobacter capsulatus ATCC BAA-309 | - |
bacteriochlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster + ADP + phosphate | - |
? | |
3-acetyl-3-devinylchlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + ATP + H2O + 2 H+ | Rhodobacter capsulatus SB1003 | - |
bacteriochlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster + ADP + phosphate | - |
? | |
8-vinyl chlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ | Rhodobacter capsulatus | - |
chlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster | - |
? | |
8-vinyl chlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ | Rhodobacter capsulatus NBRC 16581 | - |
chlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster | - |
? | |
8-vinyl chlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ | Rhodobacter capsulatus ATCC BAA-309 | - |
chlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster | - |
? | |
8-vinyl chlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ | Rhodobacter capsulatus SB1003 | - |
chlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodobacter capsulatus | P26177 | gene bchX | - |
Rhodobacter capsulatus ATCC BAA-309 | P26177 | gene bchX | - |
Rhodobacter capsulatus NBRC 16581 | P26177 | gene bchX | - |
Rhodobacter capsulatus SB1003 | P26177 | gene bchX | - |
Purification (Comment) | Organism |
---|---|
copurification of recombinant GST-tagged BchX and BchY from Escherichia coli by glutathione affinity chromatography | Rhodobacter capsulatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-acetyl-3-devinylchlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + ATP + H2O + 2 H+ | - |
Rhodobacter capsulatus | bacteriochlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster + ADP + phosphate | - |
? | |
3-acetyl-3-devinylchlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + ATP + H2O + 2 H+ | - |
Rhodobacter capsulatus NBRC 16581 | bacteriochlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster + ADP + phosphate | - |
? | |
3-acetyl-3-devinylchlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + ATP + H2O + 2 H+ | - |
Rhodobacter capsulatus ATCC BAA-309 | bacteriochlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster + ADP + phosphate | - |
? | |
3-acetyl-3-devinylchlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + ATP + H2O + 2 H+ | - |
Rhodobacter capsulatus SB1003 | bacteriochlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster + ADP + phosphate | - |
? | |
8-vinyl chlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ | - |
Rhodobacter capsulatus | chlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster | - |
? | |
8-vinyl chlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ | - |
Rhodobacter capsulatus NBRC 16581 | chlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster | - |
? | |
8-vinyl chlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ | - |
Rhodobacter capsulatus ATCC BAA-309 | chlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster | - |
? | |
8-vinyl chlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ | - |
Rhodobacter capsulatus SB1003 | chlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster | - |
? | |
additional information | enzyme COR has an alternative 8VR activity in addition to the B-ring C7=C8 double bond reduction activity, cf. EC 1.3.7.13 or EC 1.3.1.75 | Rhodobacter capsulatus | ? | - |
- |
|
additional information | enzyme COR has an alternative 8VR activity in addition to the B-ring C7=C8 double bond reduction activity, cf. EC 1.3.7.13 or EC 1.3.1.75 | Rhodobacter capsulatus NBRC 16581 | ? | - |
- |
|
additional information | enzyme COR has an alternative 8VR activity in addition to the B-ring C7=C8 double bond reduction activity, cf. EC 1.3.7.13 or EC 1.3.1.75 | Rhodobacter capsulatus ATCC BAA-309 | ? | - |
- |
|
additional information | enzyme COR has an alternative 8VR activity in addition to the B-ring C7=C8 double bond reduction activity, cf. EC 1.3.7.13 or EC 1.3.1.75 | Rhodobacter capsulatus SB1003 | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
More | In contrast to BciA and BciB, COR is a nitrogenase-like complex enzyme consisting of two separable components, the X protein (a BchX dimer) and the YZ protein (a BchY-BchZ heterotetramer). The X protein serves as the reductase component by transferring electrons from a [4Fe-4S] cluster held in the interface between the BchX homodimer to the YZ protein. The YZ protein is the catalytic component, as it accepts electrons from the X protein and transfers them to the Chlide substrate via a [4Fe-4S] cluster, to produce 3-vinyl bacteriochlorophyllide a (3 V-BChlide) | Rhodobacter capsulatus |
Synonyms | Comment | Organism |
---|---|---|
chlorophyllide a oxidoreductase | - |
Rhodobacter capsulatus |
COR | - |
Rhodobacter capsulatus |
COR 8VR | - |
Rhodobacter capsulatus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00027 | - |
8-vinyl chlorophyllide a | recombinant enzyme BchX/BchY, pH and temperature not specified in the publication | Rhodobacter capsulatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Rhodobacter capsulatus | |
Fe-S center | a [4Fe-4S] cluster is bound at the interface between the BchX homodimer and the YZ protein of the COR enzyme complex | Rhodobacter capsulatus | |
Ferredoxin | - |
Rhodobacter capsulatus |
General Information | Comment | Organism |
---|---|---|
metabolism | bacteriochlorophyll a (BChl) is an essential pigment for anoxygenic photosynthesis. In late steps of the BChl biosynthesis of Rhodobacter capsulatus, the C8 vinyl group and C7=C8 double bond of 8-vinyl chlorophyllide a (8 V-Chlide) are reduced by a C8 vinyl reductase (8VR), BciA (EC 1.3.1.75) or BciB (EC 1.3.7.13), and a nitrogenase-like enzyme, chlorophyllide a oxidoreductase (COR), respectively, to produce 3-vinyl-bacteriochlorphyllide a. BciB is a FAD-containing FeS protein that uses reduced ferredoxin as the reductant for the 8-vinyl reduction of 8 V-Chlide or 8 V-PChlide, while BciA utilizes NADPH | Rhodobacter capsulatus |
physiological function | bacteriochlorophyll a (BChl) is an essential pigment for anoxygenic photosynthesis. In late steps of the BChl biosynthesis of Rhodobacter capsulatus, the C8 vinyl group and C7=C8 double bond of 8-vinyl chlorophyllide a (8 V-Chlide) are reduced by a C8 vinyl reductase (8VR), BciA (EC 1.3.1.75) or BciB (EC 1.3.7.13), and a nitrogenase-like enzyme, chlorophyllide a oxidoreductase (COR), respectively, to produce 3-vinyl-bacteriochlorphyllide a. The COR activity can act as a third 8VR, confirmed in vivo by the loss of 8VR activity mutants lacking bciA and genes encoding subunits of COR. In contrast to BciA and BciB, COR is a nitrogenase-like complex enzyme consisting of two separable components, the X protein (a BchX dimer) and the YZ protein (a BchY-BchZ heterotetramer). The X protein serves as the reductase component by transferring electrons from a [4Fe-4S] cluster held in the interface between the BchX homodimer and the YZ protein. The YZ protein is the catalytic component, as it accepts electrons from the X protein and transfers them to the Chlide substrate via a [4Fe-4S] cluster, to produce 3-vinyl bacteriochlorophyllide a (3 V-BChlide). 3 V-BChlide is produced via Chlide rather than via 3,8-divinyl BChlide (81,82-didehydro-3 V-BChlide) | Rhodobacter capsulatus |